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Rebecca Chambers was a former rookie officer of S.T.A.R.S. (Special Tactics and Rescue Service) assigned to Bravo Team under the position of RS (Rear Security). She was in charge of medical needs and possessed extensive knowledge related to chemistry. She was an eccentric person and although she was an action type and little upset her, she had no real combat experience. She often hesitated when trying to act boldly and when overwhelmed by a situation, she couldn't help but expose her lack of experience. She gained her first combat experience during the investigation into the bizarre murders in the Arklay Mountains. She was an avid basketball fan and attended games with her friends on holidays.

The G-virus (short for "God virus") was a powerful virus developed by Dr. William Birkin with potential surpassing that of the t-virus. Its main function was the creation of "G-Creatures", a new life form designed for use as a B.O.W. (Bio Organic Weapon) that could breed through sex. However, at some point during its development, its focus turned to its potential for creating "G-Humans", a superior human race. Following its recovery from Raccoon City, it fell into the hands of several organizations.

Albert Wesker was one of the most promising subjects of the Wesker Project. Following his education, he became a Chief Researcher and prospective executive of Umbrella Corporation. He was an expert in bioengineering and became directly involved with research on the t-virus. He later became the leader of S.T.A.R.S. as Alpha Team Captain in Raccoon City.