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Jill Valentine was a former United States Army soldier, former officer of S.T.A.R.S. (Special Tactics and Rescue Service) and a founding agent of the BSAA (Bioterrorism Security Assessment Alliance).

She was a bomb disposal expert skilled with her fingers who also excelled in lock-picking and disarming traps, with general knowledge on chemical and drug related matters. She was a serious woman with very strong senses of justice and responsibility and would question without hesitation to the point of absurdity, holding on for the sake of the truth even if she were denied it.

The Progenitor virus (also known as the Clay virus) was a unique mutagenic retrovirus, a type of RNA virus discovered from a rare flower in West Africa. It possessed the ability to parasitize a host at a cellular level and mutate DNA and recombine genes, thereby strengthening a host's characteristics but causing detrimental mutations when cells developed abnormally. It was unknown to most of human civilization until its discovery by the founders of the Umbrella Corporation, who kept its existence top-secret and applied it to "B.O.W. (Bio Organic Weapons)" development, living biological weapons for military application.

Raccoon City was an urban community located in the American mid-west. At its peak, it had a population of over one hundred thousand. While existing as a town initially, it was thanks to the many donations by Umbrella that Raccoon prospered into a full fledged city in the late 20th century. Following the T-virus outbreak that afflicted the city in September 1998, the entire community was destroyed by an experimental nuclear weapon by the United States government, as part of Mission Code: XX (Bacillus Terminate).

The U.B.C.S. (Umbrella Biohazard Countermeasure Service) was a professional special operations unit privately owned by the Umbrella Corporation as a component of its security department. Its main duty was to serve as "cleaners" in situations involving the company's illegal biological and chemical weapons, such as virus leaks. Its second function was as a corporate counter-terrorism measure. Additionally, the unit is also used at times to conduct performance analysis and collect combat effectiveness data on the various weapons developed by Umbrella. 1

Leon Scott Kennedy was an American agent and former police officer. He survived the viral outbreak incident in Raccoon City and became involved in activities focused on destroying the Umbrella Corporation, harboring an intense resentment for bioterrorism. He had a strong sense of justice and put the safety of others above his own with excellent physical strength and decision-making ability. After receiving special training as an agent, he displayed a great degree of comfort in dangerous situations by telling jokes on top of his general lack of fear.